Recombinant:Article Title: Proteolytic profiling of human plasma reveals an immunoactive complement C3 fragment.
Article Snippet: .. Reagents and tools table Reagent/Resource Reference or Source Identifier or Catalog Number Experimental models N/A Human kidney organoids (Lassé et al, 2023) HEK-Blue IL6 reporter line Invivogen #hkb-hil6 U-2OS ATCC #HTB-96 Recombinant DNA N/A Antibodies N/A Monoclonal IgG anti-human CD62L BD Pharmingen Clone: DREG-56, #559772 Biotinylated antihC3d Dako #A0063 hC3Nb2 anti-C3 nanobody (Pedersen et al, 2020b) hC3Nb3 anti-C345c nanobody (Pedersen et al, 2020a) Oligonucleotides and other sequence-based reagents C3-LHF recombinant protein C3:LHF1 sequence presented Identified in Material and Methods MGWSCIILFLVATATGVHSAEENC FIQKSDDKVTLEERL-DKACEPGVDY VYKTRLVKVQLSNDFDEYIMAIEQTI KSGSDEVQVGQQRTFISPIKCREALK LEEKKHYLMWGLSSDFWGEKPNL SYIIGKDTWVEHWPEEDECQDEE NQKQCQDLGAFTESMVVFGCP NHHHHHH PR O TE O LY TO TY PE 7 eGFR 1 CXCL10 Multi-Omics Factor AnalysisPatients with active lupus nephritis vs. remission SLE N-termini (n=11923) IM M U N O A C TI VI TY Complement self-regulation MBL pathway (i)C3b deposition IL6ST binding & signaling JAK3-STAT3 signaling STING-CXCL10 release C3-LHF1 free stable complexed Figure 8. ..
Sequencing:Article Title: Proteolytic profiling of human plasma reveals an immunoactive complement C3 fragment.
Article Snippet: .. Reagents and tools table Reagent/Resource Reference or Source Identifier or Catalog Number Experimental models N/A Human kidney organoids (Lassé et al, 2023) HEK-Blue IL6 reporter line Invivogen #hkb-hil6 U-2OS ATCC #HTB-96 Recombinant DNA N/A Antibodies N/A Monoclonal IgG anti-human CD62L BD Pharmingen Clone: DREG-56, #559772 Biotinylated antihC3d Dako #A0063 hC3Nb2 anti-C3 nanobody (Pedersen et al, 2020b) hC3Nb3 anti-C345c nanobody (Pedersen et al, 2020a) Oligonucleotides and other sequence-based reagents C3-LHF recombinant protein C3:LHF1 sequence presented Identified in Material and Methods MGWSCIILFLVATATGVHSAEENC FIQKSDDKVTLEERL-DKACEPGVDY VYKTRLVKVQLSNDFDEYIMAIEQTI KSGSDEVQVGQQRTFISPIKCREALK LEEKKHYLMWGLSSDFWGEKPNL SYIIGKDTWVEHWPEEDECQDEE NQKQCQDLGAFTESMVVFGCP NHHHHHH PR O TE O LY TO TY PE 7 eGFR 1 CXCL10 Multi-Omics Factor AnalysisPatients with active lupus nephritis vs. remission SLE N-termini (n=11923) IM M U N O A C TI VI TY Complement self-regulation MBL pathway (i)C3b deposition IL6ST binding & signaling JAK3-STAT3 signaling STING-CXCL10 release C3-LHF1 free stable complexed Figure 8. ..
Biomarker Discovery:Article Title: Proteolytic profiling of human plasma reveals an immunoactive complement C3 fragment.
Article Snippet: .. Reagents and tools table Reagent/Resource Reference or Source Identifier or Catalog Number Experimental models N/A Human kidney organoids (Lassé et al, 2023) HEK-Blue IL6 reporter line Invivogen #hkb-hil6 U-2OS ATCC #HTB-96 Recombinant DNA N/A Antibodies N/A Monoclonal IgG anti-human CD62L BD Pharmingen Clone: DREG-56, #559772 Biotinylated antihC3d Dako #A0063 hC3Nb2 anti-C3 nanobody (Pedersen et al, 2020b) hC3Nb3 anti-C345c nanobody (Pedersen et al, 2020a) Oligonucleotides and other sequence-based reagents C3-LHF recombinant protein C3:LHF1 sequence presented Identified in Material and Methods MGWSCIILFLVATATGVHSAEENC FIQKSDDKVTLEERL-DKACEPGVDY VYKTRLVKVQLSNDFDEYIMAIEQTI KSGSDEVQVGQQRTFISPIKCREALK LEEKKHYLMWGLSSDFWGEKPNL SYIIGKDTWVEHWPEEDECQDEE NQKQCQDLGAFTESMVVFGCP NHHHHHH PR O TE O LY TO TY PE 7 eGFR 1 CXCL10 Multi-Omics Factor AnalysisPatients with active lupus nephritis vs. remission SLE N-termini (n=11923) IM M U N O A C TI VI TY Complement self-regulation MBL pathway (i)C3b deposition IL6ST binding & signaling JAK3-STAT3 signaling STING-CXCL10 release C3-LHF1 free stable complexed Figure 8. ..
Binding Assay:Article Title: Proteolytic profiling of human plasma reveals an immunoactive complement C3 fragment.
Article Snippet: .. Reagents and tools table Reagent/Resource Reference or Source Identifier or Catalog Number Experimental models N/A Human kidney organoids (Lassé et al, 2023) HEK-Blue IL6 reporter line Invivogen #hkb-hil6 U-2OS ATCC #HTB-96 Recombinant DNA N/A Antibodies N/A Monoclonal IgG anti-human CD62L BD Pharmingen Clone: DREG-56, #559772 Biotinylated antihC3d Dako #A0063 hC3Nb2 anti-C3 nanobody (Pedersen et al, 2020b) hC3Nb3 anti-C345c nanobody (Pedersen et al, 2020a) Oligonucleotides and other sequence-based reagents C3-LHF recombinant protein C3:LHF1 sequence presented Identified in Material and Methods MGWSCIILFLVATATGVHSAEENC FIQKSDDKVTLEERL-DKACEPGVDY VYKTRLVKVQLSNDFDEYIMAIEQTI KSGSDEVQVGQQRTFISPIKCREALK LEEKKHYLMWGLSSDFWGEKPNL SYIIGKDTWVEHWPEEDECQDEE NQKQCQDLGAFTESMVVFGCP NHHHHHH PR O TE O LY TO TY PE 7 eGFR 1 CXCL10 Multi-Omics Factor AnalysisPatients with active lupus nephritis vs. remission SLE N-termini (n=11923) IM M U N O A C TI VI TY Complement self-regulation MBL pathway (i)C3b deposition IL6ST binding & signaling JAK3-STAT3 signaling STING-CXCL10 release C3-LHF1 free stable complexed Figure 8. ..
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